Aflatoxins, produced by Aspergillus spp., are strongly toxic and carcinogenic fungal secondary metabolites. Aflatoxin biosynthesis is a complex process and involves at least 30 genes clustered within an approximately 75 kB gene cluster. In this paper, we reviewed current status of the researches on the characterized structural genes involved in aflatoxin biosynthesis and their roles in aflatoxin-producing fungi, especially in A. flavus and A. parasiticus, which will improve our understanding of the mechanism of aflatoxin biosynthesis and regulation and provide reference for further study.
Massey TE, Stewart RK, Daniels JM, Liu L. Biochemical and molecular aspects of mammalian susceptibility to aflatoxin B1 carcinogenicity. Proc Soc Exp Biol Med. 1995; 208: 213-227. Ref.: https://goo.gl/d9y8sT
Yu J, Chang PK, Cary JW, Wright M, Bhatnagar D, et al. Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus. Appl Environ Microbiol. 1995; 61: 2365-2371. Ref.: https://goo.gl/QAQBW8
Yu J. Current understanding on aflatoxin biosynthesis and future perspective in reducing aflatoxin contamination. Toxins (Basel). 2012; 4: 1024-1057. Ref.: https://goo.gl/CZ694W
Yabe K, Ando Y, Hamasaki T. Biosynthetic relationship among aflatoxins B1, B2, G1, and G2. Appl Environ Microbiol. 1988; 54: 2101-2106. Ref.: https://goo.gl/PLn7Uw
Trucksess MW, Mislivec PB, Young K, Bruce VR, Page SW. Cyclopiazonic acid production by cultures of Aspergillus and Penicillium species isolated from dried beans, corn meal, macaroni, and pecans. J Assoc Off Anal Chem. 1987; 70: 123-126. Ref.: https://goo.gl/LbmR4V
Rosfarizan M, Ariff AB, Hassan MA, Karim MI. Kojic acid production by Aspergillus flavus using gelatinized and hydrolyzed sago starch as carbon sources. Folia Microbiol (Praha). 1998; 43: 459-464. Ref.: https://goo.gl/TfxyP6
Ehrlich KC. Predicted roles of the uncharacterized clustered genes in aflatoxin biosynthesis. Toxins (Basel). 2009; 1: 37-58. Ref.: https://goo.gl/rsMKXe
Watanabe CM, Wilson D, Linz JE, Townsend CA. Demonstration of the catalytic roles and evidence for the physical association of type I fatty acid synthases and a polyketide synthase in the biosynthesis of aflatoxin B1. Chem Biol. 1996; 3: 463-469. Ref.: https://goo.gl/bjsyQ1
Watanabe CM, Townsend CA. Initial characterization of a type I fatty acid synthase and polyketide synthase multienzyme complex NorS in the biosynthesis of aflatoxin B(1). Chem Biol. 2002; 9: 981-988. Ref.: https://goo.gl/q7jwaT
Hitchman TS, Schmidt EW, Trail F, Rarick MD, Linz JE, et al. Hexanoate synthase, a specialized type I fatty acid synthase in aflatoxin B1 biosynthesis. Bioorg Chem. 2001; 29: 293-307. Ref.: https://goo.gl/YfrbLn
Townsend CA, Christensen SB, Trautwein K. Hexanoate as a starter unit in polyketide biosynthesis. J Am Chem Soc. 1984; 106: 3868-3869. Ref.: https://goo.gl/Ua2fQB
Mahanti N, Bhatnagar D, Cary JW, Joubran J, Linz JE. Structure and function of fas-1A, a gene encoding a putative fatty acid synthetase directly involved in aflatoxin biosynthesis in Aspergillus parasiticus. Appl Environ Microbiol. 1996; 62: 191-195. Ref.: https://goo.gl/DeXEb9
Hong SY, Roze LV, Wee J, Linz JE. Evidence that a transcription factor regulatory network coordinates oxidative stress response and secondary metabolism in aspergilli. Microbiologyopen. 2013; 2: 144-160. Ref.: https://goo.gl/AkvVWj
Roze LV, Miller MJ, Rarick M, Mahanti N, Linz JE. A novel cAMP-response element, CRE1, modulates expression of nor-1 in Aspergillus parasiticus. J Biol Chem. 2004; 279: 27428-27439. Ref.: https://goo.gl/BUJME6
Crawford JM, Dancy BC, Hill EA, Udwary DW, Townsend CA. Identification of a starter unit acyl-carrier protein transacylase domain in an iterative type I polyketide synthase. Proc Natl Acad Sci U S A. 2006; 103: 16728-16733. Ref.: https://goo.gl/R1JscB
Crawford JM, Korman TP, Labonte JW, Vagstad AL, Hill EA, et al. Structural basis for biosynthetic programming of fungal aromatic polyketide cyclization. Nature. 2009; 461: 1139-1143. Ref.: https://goo.gl/95sTJk
Chang PK, Cary JW, Yu J, Bhatnagar D, Cleveland TE. The Aspergillus parasiticus polyketide synthase gene pksA, a homolog of Aspergillus nidulans wA, is required for aflatoxin B1 biosynthesis. Mol Gen Genet. 1995; 248: 270-277. Ref.: https://goo.gl/b2iYwm
Trail F, Mahanti N, Rarick M, Mehigh R, Liang SH, et al. Physical and transcriptional map of an aflatoxin gene cluster in Aspergillus parasiticus and functional disruption of a gene involved early in the aflatoxin pathway. Appl Environ Microbiol. 1995; 61: 2665-2673. Ref.: https://goo.gl/nkPc5w
Feng GH, Leonard TJ. Characterization of the polyketide synthase gene (pksL1) required for aflatoxin biosynthesis in Aspergillus parasiticus. J Bacteriol. 1995; 177: 6246-6254. Ref.: https://goo.gl/M4Kgf7
Ehrlich KC, Montalbano BG, Cary JW. Binding of the C6-zinc cluster protein, AFLR, to the promoters of aflatoxin pathway biosynthesis genes in Aspergillus parasiticus. Gene. 1999; 230: 249-257. Ref.: https://goo.gl/93LpKG
Ehrlich KC, Montalbano BG, Cary JW, Cotty PJ. Promoter elements in the aflatoxin pathway polyketide synthase gene. Biochim Biophys Acta. 2002; 1576: 171-175. Ref.: https://goo.gl/GW2XiA
Udwary DW, Merski M, Townsend CA. A method for prediction of the locations of linker regions within large multifunctional proteins, and application to a type I polyketide synthase. J Mol Biol. 2002; 323: 585-598. Ref.: https://goo.gl/P7gSqS
Crawford JM, Vagstad AL, Ehrlich KC, Townsend CA. Starter unit specificity directs genome mining of polyketide synthase pathways in fungi. Bioorg Chem. 2008; 36: 16-22. Ref.: https://goo.gl/e7uUnF
Crawford JM, Thomas PM, Scheerer JR, Vagstad AL, Kelleher NL, et al. Deconstruction of iterative multidomain polyketide synthase function. Science. 2008; 320: 243-246. Ref.: https://goo.gl/XARYms
Bennett JW, Christensen SB. New perspectives on aflatoxin biosynthesis. Adv Appl Microbiol. 1983; 29: 53-92. Ref.: https://goo.gl/LdqTXq
Ehrlich KC, Scharfenstein LL Jr., Montalbano BG, Chang PK. Are the genes nadA and norB involved in formation of aflatoxin G(1)? Int J Mol Sci. 2008; 9: 1717-1729. Ref.: https://goo.gl/V8fJv4
Zhou R, Linz JE. Enzymatic function of the nor-1 protein in aflatoxin biosynthesis in Aspergillus parasiticus. Appl Environ Microbiol. 1999; 65: 5639-5641. Ref.: https://goo.gl/8VYKGB
Trail F, Chang PK, Cary J, Linz JE. Structural and functional analysis of the nor-1 gene involved in the biosynthesis of aflatoxins by Aspergillus parasiticus. Appl Environ Microbiol. 1994; 60: 4078-4085. Ref.: https://goo.gl/bvqnoQ
Miller MJ, Roze LV, Trail F, Linz JE. Role of cis-acting sites NorL, a TATA box, and AflR1 in nor-1 transcriptional activation in Aspergillus parasiticus. Appl Environ Microbiol. 2005; 71: 1539-1545. Ref.: https://goo.gl/YwkVTu
Yabe K, Nakamura Y, Nakajima H, Ando Y, Hamasaki T. Enzymatic conversion of norsolorinic acid to averufin in aflatoxin biosynthesis. Appl Environ Microbiol. 1991; 57: 1340-1345. Ref.: https://goo.gl/T1SZo4
Cary JW, Wright M, Bhatnagar D, Lee R, Chu FS. Molecular characterization of an Aspergillus parasiticus dehydrogenase gene, norA, located on the aflatoxin biosynthesis gene cluster. Appl Environ Microbiol. 1996; 62: 360-366. Ref.: https://goo.gl/VA1iw1
Yu J, Bhatnagar D, Cleveland TE. Completed sequence of aflatoxin pathway gene cluster in Aspergillus parasiticus. FEBS Lett. 2004; 564: 126-130. Ref.: https://goo.gl/MEzEze
Ehrlich KC, Chang PK, Yu J, Cotty PJ. Aflatoxin biosynthesis cluster gene cypA is required for G aflatoxin formation. Appl Environ Microbiol. 2004; 70: 6518-6524. Ref.: https://goo.gl/Ukixmd
Chang PK, Yu J, Ehrlich KC, Boue SM, Montalbano BG, et al. adhA in Aspergillus parasiticus is involved in conversion of 5'-hydroxyaverantin to averufin. Appl Environ Microbiol. 2000; 66: 4715-4719. Ref.: https://goo.gl/q8HbAL
Bateman A, Birney E, Durbin R, Eddy SR, Finn RD, et al. Pfam 3.1: 1313 multiple alignments and profile HMMs match the majority of proteins. Nucleic Acids Res. 1999; 27: 260-262. Ref.: https://goo.gl/DvrSZ5
Sakuno E, Yabe K, Nakajima H. Involvement of two cytosolic enzymes and a novel intermediate, 5'-Oxoaverantin, in the pathway from 5'-Hydroxyaverantin to averufin in aflatoxin biosynthesis. Appl Environ Microbiol. 2003; 69: 6418-6426. Ref.: https://goo.gl/uJYiWt
Yu J, Woloshuk CP, Bhatnagar D, Cleveland TE. Cloning and characterization of avfA and omtB genes involved in aflatoxin biosynthesis in three Aspergillus species. Gene. 2000; 248: 157-167. Ref.: https://goo.gl/1LwU74
Motomura M, Chihaya N, Shinozawa T, Hamasaki T, Yabe K. Cloning and characterization of the O-methyltransferase I gene (dmtA) from Aspergillus parasiticus associated with the conversions of demethylsterigmatocystin to sterigmatocystin and dihydrodemethylsterigmatocystin to dihydrosterigmatocystin in aflatoxin biosynthesis. Appl Environ Microbiol. 1999; 65: 4987-4994. Ref.: https://goo.gl/oSedyJ
Yabe K, Chihaya N, Hamamatsu S, Sakuno E, Hamasaki T, et al. Enzymatic conversion of averufin to hydroxyversicolorone and elucidation of a novel metabolic grid involved in aflatoxin biosynthesis. Appl Environ Microbiol. 2003; 69: 66-73. Ref.: https://goo.gl/Zry8nN
Yu J, Chang PK, Bhatnagar D, Cleveland TE. Genes encoding cytochrome P450 and monooxygenase enzymes define one end of the aflatoxin pathway gene cluster in Aspergillus parasiticus. Appl Microbiol Biotechnol. 2000; 53: 583-590. Ref.: https://goo.gl/cyqcvZ
Cary JW, Ehrlich KC, Bland JM, Montalbano BG. The aflatoxin biosynthesis cluster gene, aflX, encodes an oxidoreductase involved in conversion of versicolorin A to demethylsterigmatocystin. Appl Environ Microbiol. 2006; 72: 1096-1101. Ref.: https://goo.gl/CG9Sxb
Ehrlich KC, Montalbano B, Boue SM, Bhatnagar D. An aflatoxin biosynthesis cluster gene encodes a novel oxidase required for conversion of versicolorin a to sterigmatocystin. Appl Environ Microbiol. 2005; 71: 8963-8965. Ref.: https://goo.gl/iPggiS
Liang SH, Skory CD, Linz JE. Characterization of the function of the ver-1A and ver-1B genes, involved in aflatoxin biosynthesis in Aspergillus parasiticus. Appl Environ Microbiol. 1996; 62: 4568-4575. Ref.: https://goo.gl/uUepJY
Skory CD, Chang PK, Cary J, Linz JE. Isolation and characterization of a gene from Aspergillus parasiticus associated with the conversion of versicolorin A to sterigmatocystin in aflatoxin biosynthesis. Appl Environ Microbiol. 1992; 58: 3527-3537. Ref.: https://goo.gl/YVtkf2
Hallam SE, Malpartida F, Hopwood DA. Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor. Gene. 1988; 74: 305-320. Ref.: https://goo.gl/EPXthu
Delledonne M, Porcari R, Fogher C. Nucleotide sequence of the nodG gene of Azospirillum brasilense. Nucleic Acids Res. 1990; 18: 6435. Ref.: https://goo.gl/6G4AsR
Heilmann HJ, Magert HJ, Gassen HG. Identification and isolation of glucose dehydrogenase genes of Bacillus megaterium M1286 and their expression in Escherichia coli. Eur J Biochem. 1988; 174: 485-490. Ref.: https://goo.gl/rUd2k4
Hopwood DA, Sherman DH. Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Annu Rev Genet. 1990; 24: 37-66. Ref.: https://goo.gl/1AwzYP
Kamps MP, Taylor SS, Sefton BM. Direct evidence that oncogenic tyrosine kinases and cyclic AMP-dependent protein kinase have homologous ATP-binding sites. Nature. 1984; 310: 589-592. Ref.: https://goo.gl/R3KUKy
Udwary DW, Casillas LK, Townsend CA. Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis. J Am Chem Soc. 2002; 124: 5294-5303. Ref.: https://goo.gl/2ju9Au
Price MS, Yu J, Nierman WC, Kim HS, Pritchard B, et al. The aflatoxin pathway regulator AflR induces gene transcription inside and outside of the aflatoxin biosynthetic cluster. FEMS Microbiol Lett. 2006; 255: 275-279. Ref.: https://goo.gl/kmx4hm
Yu J, Chang PK, Cary JW, Bhatnagar D, Cleveland TE. avnA, a gene encoding a cytochrome P-450 monooxygenase, is involved in the conversion of averantin to averufin in aflatoxin biosynthesis in Aspergillus parasiticus. Appl Environ Microbiol. 1997; 63: 1349-1356. Ref.: https://goo.gl/4emi7c
Cary JW, Montalbano BG, Ehrlich KC. Promoter elements involved in the expression of the Aspergillus parasiticus aflatoxin biosynthesis pathway gene avnA. Biochim Biophys Acta. 2000; 1491: 7-12. Ref.: https://goo.gl/oapVdn
Keller NP, Watanabe CM, Kelkar HS, Adams TH, Townsend CA. Requirement of monooxygenase-mediated steps for sterigmatocystin biosynthesis by Aspergillus nidulans. Appl Environ Microbiol. 2000; 66: 359-362. Ref.: https://goo.gl/iwAA8S
Yabe K, Hamasaki T. Stereochemistry during aflatoxin biosynthesis: cyclase reaction in the conversion of versiconal to versicolorin B and racemization of versiconal hemiacetal acetate. Appl Environ Microbiol. 1993; 59: 2493-2500. Ref.: https://goo.gl/dr19gi
Bhatnagar D, Cleveland TE, Kingston DG. Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis. Biochemistry. 1991; 30: 4343-4350. Ref.: https://goo.gl/KMPVYv
Yabe K, Ando Y, Hamasaki T. Desaturase activity in the branching step between aflatoxins B1 and G1 and aflatoxins B2 and G2. Agric Biol Chem. 1991; 55: 1907-1911. Ref.: https://goo.gl/t6DqxM
Keller NP, Segner S, Bhatnagar D, Adams TH. stcS, a putative P-450 monooxygenase, is required for the conversion of versicolorin A to sterigmatocystin in Aspergillus nidulans. Appl Environ Microbiol. 1995; 61: 3628-3632. Ref.: https://goo.gl/c76Qba
Calvo AM, Wilson RA, Bok JW, Keller NP. Relationship between secondary metabolism and fungal development. Microbiol Mol Biol Rev. 2002; 66: 447-459. Ref.: https://goo.gl/BAePgG
Reiss J. Development of Aspergillus parasiticus and formation of aflatoxin B1 under the influence of conidiogenesis affecting compounds. Arch Microbiol. 1982; 133: 236-238. Ref.: https://goo.gl/KQR3cu
Wieser J, Yu JH, Adams TH. Dominant mutations affecting both sporulation and sterigmatocystin biosynthesis in Aspergillus nidulans. Curr Genet. 1997; 32: 218-224. Ref.: https://goo.gl/bQdk9g
Prieto R, Yousibova GL, Woloshuk CP. Identification of aflatoxin biosynthesis genes by genetic complementation in an Aspergillus flavus mutant lacking the aflatoxin gene cluster. Appl Environ Microbiol. 1996; 62: 3567-3571. Ref.: https://goo.gl/SbGCgC
Yu J, Chang PK, Ehrlich KC, Cary JW, Montalbano B, et al. Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2. Appl Environ Microbiol. 1998; 64: 4834-4841. Ref.: https://goo.gl/CAFAPM
Prieto R, Woloshuk CP. ord1, an oxidoreductase gene responsible for conversion of O-methylsterigmatocystin to aflatoxin in Aspergillus flavus. Appl Environ Microbiol. 1997; 63: 1661-1666. Ref.: https://goo.gl/7Huf4a
Cai J, Zeng H, Shima Y, Hatabayashi H, Nakagawa H, et al. Involvement of the nadA gene in formation of G-group aflatoxins in Aspergillus parasiticus. Fungal Genet Biol. 2008; 45: 1081-1093. Ref.: https://goo.gl/y6VNYo
Yu J, Chang P, Bhatnagar D, Cleveland TE. Cloning of a sugar utilization gene cluster in Aspergillus parasiticus. Biochim Biophys Acta. 2000; 1493: 211-214. Ref.: https://goo.gl/u8AkSx
Fox KM, Karplus PA. Old yellow enzyme at 2 Å resolution: overall structure, ligand binding, and comparison with related flavoproteins. Structure. 1994; 2: 1089-1105. Ref.: https://goo.gl/oEWSf6
Williams RE, Bruce NC. 'New uses for an Old Enzyme'--the Old Yellow Enzyme family of flavoenzymes. Microbiology. 2002; 148: 1607-1614. Ref.: https://goo.gl/QRPEoA
Sakuno E, Wen Y, Hatabayashi H, Arai H, Aoki C, et al. Aspergillus parasiticus cyclase catalyzes two dehydration steps in aflatoxin biosynthesis. Appl Environ Microbiol. 2005; 71: 2999-3006. Ref.: https://goo.gl/doux9q
McGuire SM, Silva JC, Casillas EG, Townsend CA. Purification and characterization of versicolorin B synthase from Aspergillus parasiticus. Catalysis of the stereodifferentiating cyclization in aflatoxin biosynthesis essential to DNA interaction. Biochemistry. 1996; 35: 11470-11486. Ref.: https://goo.gl/A3KPSS
Lin BK, Anderson JA. Purification and properties of versiconal cyclase from Aspergillus parasiticus. Arch Biochem Biophys. 1992; 293: 67-70. Ref.: https://goo.gl/XHcZKv
Silva JC, Minto RE, Barry CE 3rd, Holland KA, Townsend CA. Isolation and characterization of the versicolorin B synthase gene from Aspergillus parasiticus. Expansion of the aflatoxin b1 biosynthetic gene cluster. J Biol Chem. 1996; 271: 13600-13608. Ref.: https://goo.gl/rjgw9B
Silva JC, Townsend CA. Heterologous expression, isolation, and characterization of versicolorin B synthase from Aspergillus parasiticus. A key enzyme in the aflatoxin B1 biosynthetic pathway. J Biol Chem. 1997; 272: 804-813. Ref.: https://goo.gl/Vgk1c4
Juvvadi PR, Chivukula S. Putative calmodulin-binding domains in aflatoxin biosynthesis-regulatory proteins. Curr Microbiol. 2006; 52: 493-496. Ref.: https://goo.gl/Zn1k4d
Hsieh DP, Wan CC, Billington JA. A versiconal hemiacetal acetate converting enzyme in aflatoxin biosynthesis. Mycopathologia. 1989; 107: 121-126. Ref.: https://goo.gl/vBLdB5
Kusumoto K, Hsieh DP. Purification and characterization of the esterases involved in aflatoxin biosynthesis in Aspergillus parasiticus. Can J Microbiol. 1996; 42: 804-810. Ref.: https://goo.gl/bUqseb
Yu J, Chang PK, Bhatnagar D, Cleveland TE. Cloning and functional expression of an esterase gene in Aspergillus parasitcus. Mycopathologia. 2002; 156: 227-234. Ref.: https://goo.gl/8uP3Dv
Yabe K, Ando Y, Hashimoto J, Hamasaki T. Two distinct O-methyltransferases in aflatoxin biosynthesis. Appl Environ Microbiol. 1989; 55: 2172-2177. Ref.: https://goo.gl/DVHfJM
Yabe K, Matsushima K, Koyama T, Hamasaki T. Purification and characterization of O-methyltransferase I involved in conversion of demethylsterigmatocystin to sterigmatocystin and of dihydrodemethylsterigmatocystin to dihydrosterigmatocystin during aflatoxin biosynthesis. Appl Environ Microbiol. 1998; 64: 166-171. Ref.: https://goo.gl/fTbHrK
Yu J, Chang PK, Payne GA, Cary JW, Bhatnagar D, et al. Comparison of the omtA genes encoding O-methyltransferases involved in aflatoxin biosynthesis from Aspergillus parasiticus and A. flavus. Gene. 1995; 163: 121-125. Ref.: https://goo.gl/DKUJvr
Yu J, Cary JW, Bhatnagar D, Cleveland TE, Keller NP, et al. Cloning and characterization of a cDNA from Aspergillus parasiticus encoding an O-methyltransferase involved in aflatoxin biosynthesis. Appl Environ Microbiol. 1993; 59: 3564-3571. Ref.: https://goo.gl/NMdDbf
Chauvaux S, Chevalier F, Le Dantec C, Fayolle F, Miras I, et al. Cloning of a genetically unstable cytochrome P-450 gene cluster involved in degradation of the pollutant ethyl tert-butyl ether by Rhodococcus ruber. J Bacteriol. 2001; 183: 6551-6557. Ref.: https://goo.gl/pE99zQ
Lin JQ, Zhao XX, Zhi QQ, Zhao M, He ZM. Transcriptomic profiling of Aspergillus flavus in response to 5-azacytidine. Fungal Genet Biol. 2013; 56:78-86. Ref.: https://goo.gl/HtSYFA
Liu SY, Lin JQ, Wu HL, Wang CC, Huang SJ, et al. Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation. PLoS ONE. 2012; 7: e30349. Ref.: https://goo.gl/7htLt5
Zhi QQ, Li JY, Liu QY, He ZM. A cytosine methyltransferase ortholog dmtA is involved in the sensitivity of Aspergillus flavus to environmental stresses. Fungal Biol. 2017; 121: 501-514. Ref.: https://goo.gl/wv4WPa
Zhi QQ, Xie YY, He ZM. Genome mining for aflatoxin biosynthesis. Fungal Genom Biol. 2013; 3: 108. Ref.: https://goo.gl/cGGv39
Zhao X, Spraker JE, Bok JW, Velk T, He ZM, Keller NP. A cellular fusion cascade regulated by LaeA is required for sclerotial development in Aspergillus flavus. Front Microbiol. 2017; 8: 1925. Ref.: https://goo.gl/N6zB77
Zhao X, Zhi QQ, Li JY, Keller NP, He ZM. The antioxidant gallic acid inhibits aflatoxin formation in Aspergillus flavus by modulating transcription factors FarB and CreA. Toxins (Basel). 2018; 10: 270. Ref.: https://goo.gl/PczwsJ
Chang PK, Skory CD, Linz JE. Cloning of a gene associated with aflatoxin B1 biosynthesis in Aspergillus parasiticus. Curr Genet. 1992; 21: 231-233. Ref.: https://goo.gl/oTTyhx
Lin JQ, Zhao XX, Wang CC, Xie Y, Li GH, et al. Transcriptomic profiling of Aspergillus flavus in response to 5-azacytidine. Ann Microbiol. 2012; 63: 763-769. Ref.: https://goo.gl/46h5qV
Nierman WC, Yu J, Fedorova-Abrams ND, Losada L, Cleveland TE, et al. Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes aflatoxin contamination of food and feed. Genome Announc. 2015; 3. Ref.: https://goo.gl/7ijZGU
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Assistant Professor, UCLAS Uttaranchal University, Dehradun, India
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New Delhi, India
Anubha Bajaj
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Andy Smith
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Publishing an article is a long process, but working with your publication department made things go smoothly, even though the process took exactly 5 months from the time of submitting the article till the time I have favourable response, the missing part is the peer review details, which is essential in self auditing and future improvement, overall experience was excellent giving your understanding of the situation of lack of financial institution support.
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Respectfully.
Dr. Alon
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Alexandra Cozma
To the editorial team at HSPI and the Journal of Clinical Nephrology:
Thank you so much for your hard work and collaboration in bringing our article to life. Your staff was responsive, flexible, and communicative and made the process smooth and easy. Thank you!
Alejandro Munoz
Dear colleagues! I am satisfied with our cooperation with you. Your service is at a high level. I hope for a future relationship. Let me know if I can get a paper version of the magazine with my articles from you. I see them on the Internet.
Aksenov V.V
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Ajite Kayode
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Achraf elbakkaly
I really liked the ease of submitting my manuscript in the HSPI journal. Further, the peer review was timely completed and I was communicated the final decision on my manuscript within 10 days of submission which is really appreciable. I strongly recommend all the scientists and researchers to submit their work in this journal”
Abu Bashar
My candid opinion is that the service you render is second to none. My favourite part is the prompt response to issue, really i value that.
Abiodun Akanbi Adeogun
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Abdul Baset
I, Muhammad Sarwar Khan, am serving as Editor on Archives of Biotechnology and Biomedicine (ABB). I submitted an editorial titled, 'Edible vaccines to combat Infectious Bursal Disease of poultry' for publication in ABB. After submitting the manuscript; the services rendered by the management and technical personnel to handle and process the manuscript were marvelous. Plagiarism report was shared with me with complements before reviewers' comments, All steps including article processing and service charges were well taken care of keeping in view the author's interest/preference. All together, it was an encouraging and wonderful experience working with ABB personnel.
University of Agriculture, Pakistan
Muhammad Sarwar Khan
Your journal has accomplished its intended mission of providing very effective and efficient goals in dealing with submissions, conducting the reviewing process and in publishing accepted manuscripts in a timely manner. Keep up the great work and services that you provide.
University of Jacqmar, Inc., USA
John St. Cyr
I am to express my view that Heighten Science Publications are reliable quick even after peer review process. I hope and wish the publications will go a long way in disseminating science to many interested in scientific research.
College of Fisheries, CAU(I), Tripura, India
Ajit Kumar Roy
The Journal Clinical Nephrology provides a good opportunity for readers to stay updated in the field of clinical nephrology. Additionally - it provides a good opportunity for authors to publish their work.
1. Publication of the accepted manuscripts is sufficiently rapid.
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3. Peer review process very rapid and effective.
Assaf Harofeh Medical Center, Israel
Leonid Feldman
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University of Tubingen, Germany
Yvonne Mast
We thank to the heighten science family, who speed up the publication of our article and provide every support.
Mehmet Besir
The services of the journal were excellent. The most important thing for an author is the speed of the peer review which was really fast here. They returned in a few days and immediately replied all of my questions. I want to refer this platform to all scholars.
Many thanks.
Eastern Mediterranean University, Cyprus
Zehra Guchan TOPCU
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USA
Igor Klepikov
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Be well all the year long.
Russia
Victor V Apollonov
The editorial process was quickly done. The galley proof was sent within a week after being accepted for publication.
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India
Rohit Kulshrestha
Publishing with the International Journal of Clinical and Experimental Ophthalmology was a rewarding experience as review process was thorough and brisk. Their visibility online is second to none as their published articles appear in all search engines. I will encourage researchers to publish with them.
University of Port Harcourt Teaching Hospital, Nigeria
Dr. Elizabeth A Awoyesuku
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NC A&T State University, USA
Moran Sciamama-Saghiv
Submission of paper was smooth, the review process was fast. I had excellent communication and on time response from the editor.
Ekiti State University Teaching Hospital, Nigeria
Ayokunle Dada
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Ido-Ekiti/Afe Babalola University, Nigeria
Dr. Shuaib Kayode Aremu
"This is my first time publishing with the journal/publisher. I am impressed at the promptness of the publishing staff and the professionalism displayed. Thank you for encouraging young researchers like me!"
Ekiti State University, Nigeria
Adebukola Ajite
I wanna to thank clinical journal of nursing care and practice for its effort to review and publish my manuscript. This is reputable journal. Thank you!
Wollo University, Ethiopia
Atsedemariam Andualem
We appreciate your approach to scholars and will encourage you to collaborate with your organization, which includes interesting and different medical journals.
With the best wishes of success, creativity and joy in life, prosperity in the medical field.
Ivano- Frankivsk National Medical University, Ukraine
Nataliya Kitsera
Thank you very much for your support and encouragement. I am truly impressed by your tolerance and support.
Thank you very much
Diaverum: PADC, Jeddah, Saudi Arabia
Nasrulla Abutaleb
You are such a nice person. Your journal co-operation is very appreciable and motivational.
Department of Biotechnology, Uttaranchal college of Applied and Life Sciences, Uttaranchal University, Dehradun, Uttarakhand, India
Archna Dhasmana
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BS, PharmD., MBA, CPHIMS, FHIMSS, Adjunct Professor, Global Healthcare Management, MCPHS University, Chief Strategy Offi cer, MedicaSoft, Senior Advisor, National Health IT (NHIT) Collaborative for Underserved, New York HIMSS, National Liaison, Health 2.0 Boston, Past Chair, Chair Innovation, USA
Helen Figge
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Ph.D, Boston University Department of Communication Sciences and Disorders and Knowledge Research Institute, Inc., 2131 Reflection Bay Drive, Arlington, Texas 76013, USA
Elisabeth H. Wiig
The service is nice and the time of processing the application is fast.
Department of Neurosurgery, Queen Elizabeth Hospital, Hong Kong
Long Ching
Your service is very good and fast reply, Also your service understand our situation and support us to publication our articles.
Palestine College of Nursing, Khan Younis, Gaza Strip, Palestine
Ayman M Abu Mustafa
“It was a delightful experience publishing my manuscript with the Clinical Journal of Obstetrics and Gynecology. They offered me lots of opportunities I never had from most publishing houses and their prompt services are greatly appreciated.”
Department of Agricultural Economics, Agribusiness and Extension, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
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