An isolate of zucchini yellow mosaic virus (ZYMV) was obtained from naturally infected squash fruits were grown in Abees region, Alexandria governorate. Disease symptoms were Showing mosaic, yellowing and blistering and absis symptoms. The identification was based on the symptoms developed on diagnostic hosts and serological reactions with antisera to cucumber mosaic cucumovirus (CMV), watermelon mosaic potyvirus 2 (WMV-2) and ZYMV. Squash fruit isolate of ZYMV was transmitted by Aphis gossypii, Aphis neri and Myzus persicae in non-persistent manner. The virus was purified by ultra-centrifugation and PEG. The purified virus had an ultraviolet absorption spectrum typical of a nucleoprotein with A260/280 and A280/260 being 1.1 and 0.91 respectively. The yield of purified virus was 1.62 mg/100g infected leaf tissues. Specific antiserum was prepared and found to have a titer of 1:409600 as determined by indirect ELISA.
Lisa V, Lecoq H. Zucchini yellow mosaic virus. CMI/AAB Description of Plant Viruses. 1984; No. 282.
Lisa V, Boccardo G, D’Agostino G, Dellavalle G, d’Aquilo M. Characterization of a potyvirus that causes zucchini yellow mosaic. Phytopathology. 1981; 71:667-672.
Lesemann DE, Makkouk KM, Koenig R, Natafji Samman E. Natural infection of cucumbers by zucchini yellow mosaic virus in Lebanon. Phytopathol Z. 1983; 108: 304-313.
Davis RF. Partial characterization of zucchini yellow mosaic virus isolated from squash in Turkey. Plant Dis. 1986; 70:735-738.
Al-Musa AM. Severe mosaic caused by zucchini yellow mosaic virus in cucurbit from Jordan. Plant Pathology. 1989; 38:541-546.
Antignus Y, Raccah B, Gal-On A. Biological and serological characterization of zucchini yellow mosaic virus and watermelon mosaic virus-2 isolates in Israel. Phytoparasitica. 1989; 17: 289-297.
Al-Shahwan IM. First report of zucchini yellow mosaic virus on cucurbits in the central region of Saudi Arabia. J. King Saud Univ. 1990; 2:251-260.
Vučurović A1, Bulajić A1, Stanković I1, Ristić D1, Nikolić D1, et al. First Report of Zucchini yellow mosaic virus in Watermelon in Serbia. Plant Dis. 2012; 96: 149. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/30731890
Khalil EM, El-Said H, Attia MF, Ibrahim IA. Occurrence of zucchini yellow mosaic virus in Egypt. The 1st Nat. Conf. of Pest, Disease of Veg. and Field Crops. Ismailia, Egypt. 1985; 672-675.
Ibrahim LM. Studies on some viruses affecting cucurbits in A.R.E. Ph. D. Thesis. Fac of Agric. Cairo Univ. Egypt. 1986; 119.
Abdel-Ghaffar MH, El-Dougdoug KA, Sadik AS. Serology and partial characterization of the Egyptian isolate of zucchini yellow mosaic potyvirus. 1998; 6: 313-327.
Farag AG. Studies on zucchini yellow mosaic virus in Egypt. 1999; 130.
Younes HAA. Natural infection of luffa (Luffa aegyptiaca. Mill) with Zucchini Yellow Mosaic Virus in Egypt. J Adv Agric Res. 2003; 8: 227–239.
Fath-Allah MM, Ahmed AA. Sensitive detection of watermelon mosaic and zucchini yellow mosaic viruses from infected squash plants using serological methods and polymerase chain reaction. Egyptian J Exper Biol. 2011; 7: 179-185.
Hamza KA, Abd El-Aziz MH, Behiry SI, Younes HA. Isolation and purification of potato virus y isolate infecting potato (Solanum tuberosum l.) in al-nubaria region. Middle East J Agri Res. 2018; 7: 1201-1207 .
Provvidenti R, Gonsalves D, Humaydan HS. Occurrence of zucchini yellow mosaic virus in cucurbits from Connenticut, New York, Florida, and California. Plant Dis. 1984; 68: 443-446.
Huang CH, Liang SC, Deng TC, Hseu SH. Comparison of diagnostic hosts and serological tests for four cucurbit potyviruses. Plant Pathology Bulletin. 1993; 2: 169-176.
Prieto, H., Bruna, A., Hinrichsen, P., and Muñoz, C. 2001. Isolation and molecular characterization of a Chilean isolate of Zucchini yellow mosaic virus. Plant Dis. 85:644-648.
Usher L, Sivparsad B, Gubba A. Isolation, identification and molecular characterization of an isolate of Zucchini yellow mosaic virus occurring in KwaZulu-Natal, South Africa. South African J Plant Soil. 2012; 29: 65–71.
Gal-On A. Zucchini yellow mosaic virus: insect transmission and pathogenicity—the tails of two proteins. Molecular Plant Pathology. 2007; 8: 139–150.
Abd El-Aziz MH. Studies on some viruses infecting cowpea plants. 2019a; 165.
Abd El-Aziz MH, Younes HA. Detection of Cucumber mosaic cucumovirus in infected cowpea plants (Vigna unguiculata L.) from northern Egypt. Novel Res Microbiol J. 2019; 3: 326 – 340 .
Abd El-Aziz MH. Three modern serological methods to detect plant viruses. J Plant Sci Phytopathol. 2019b; 3: 101-106.
Azzam OI, Makkouk KM. Purification of two potyvirus isolates infecting Phaseolus vulgaris L. in Lebanon. Phytopathological Mediterranean. 1986; 25: 125-130.
Noordam D. Identification of Plant Viruses Methods and Experiments". Centre for Agricultural Publishing and Documentation, Wageningen, the Netherlands. 1973; 207.
Abd El-Aziz MH. The Importance of Potato virus Y Potyvirus. J Plant Sci Phytopathol. 2020; 4: 009-015.
Purcifull DE, Adlerz WC, Simone GW, Hiebert E, Christie SR. Serological relationships and patial characterization of zucchini yellow mosaic virus isolated from squash in Florida. Plant Dis. 1984; 68:230-233.
Mahgoub HA, Desbiez C, Wipf-Scheibel C, Dafalla G, Lecoq H. Characterization and occurrence of zucchini yellow mosaic virus in Sudan. Plant Pathol. 1997; 46: 800-805.
Greber RS, Persley DM, Herrington ME. Some characteristics of Australian isolates of zucchini yellow mosaic virus. Aust J Agric Res. 1988; 39:1085-1094.
Bhargavi V, Gaddam SA, Kotakadi VS, Sai Gopal DVR. Partial Characterization of Zucchini yellow mosaic virus infecting Gherkin (Cucumis anguira L.) in chittoor district of ndhra Pradesh, India. Res J Pharmaceutical Biological Chem Sci. 2017; 8: 522-526.
Huang CH, Hseu SH. Purification, serology and properties of five zucchini yellow mosaic virus isolates. Plant Pathol. 1989; 38: 414-420.
Fegla GI, El-Samra IA, Younes HA, Abd El-Aziz MH. Comparative studies for detection of Tomato mosaic tobamovirus (ToMV), cucumber mosaic cucumovirus (CMV) and Potato Y potyviruses (PVY). Adv Agric Res. 2001; 6: 239–254.
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Shu Wei*, Muhammad Abdullah, Ferdinand L Shamalla and Mohammad M RanaShu Wei*,Muhammad Abdullah,Ferdinand L Shamalla,Mohammad M Rana. Wild-type Agrobacterium rhizogenes-mediated gene transfer in plants: Agrobacterium virulence and selection of transformants. . 2017 doi: 10.29328/journal.jpsp.1001005; 1: 044-051
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Rueda-Puente EO*, Renganathan P, Ruíz-Alvarado C, Hernández-Montiel LG and Prasath DuraisamyRueda-Puente EO*,Renganathan P,Ruíz-Alvarado C,Hernández-Montiel LG,Prasath Duraisamy. Evaluation of genetic diversity in germplasm of paprika (Capsicum spp.) using random amplified polymorphic DNA (RAPD) markers. . 2017 doi: 10.29328/journal.jpsp.1001010; 1: 080-086
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Hossam Aldeen Anwer and Abubakr Hassan*Hossam Aldeen Anwer and Abubakr Hassan*. Air Quality Dynamics in Sichuan Province: Sentinel-5P Data Insights (2019-2023). Ann Civil Environ Eng. 2024: doi: 10.29328/journal.acee.1001068; 8: 057-062
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