Abstract

Research Article

Nitrogen supply effect on lettuce response to Botrytis cinerea and Sclerotinia minor

Chayma Ouhibi*, Majd Darwish, Laurent Urban, Mokhtar Lachaal and Jawad Aarrouf

Published: 06 November, 2023 | Volume 7 - Issue 3 | Pages: 118-123.

Background information: Cultural practices play an important role on the development of pathogens (Datnoff, et al. 2007). In this context, several authors have been interested in studying the effects of mineral nutrition on the resistance of vegetables and fruits to fungi during storage, especially nitrogen.
The purpose of the study: In this work we tested the effects of three contrasted regimes of nitrogen supply, with nitrate concentrations in the nutrient solutions of 2 mM (low), 10 mM (normal) and 20 mM (high) on the susceptibility of Lactuca sativa L towards Botrytis cinerea (BC87) and Sclerotinia minor (SM) during storage.
Once harvested, the outerleaves of the plants derived from the three nitrogen regimes were inoculated with either Botrytis cinerea (BC87) or Sclerotinia minor (SM). Data showed that the resistance to this two pathogens increase when plants were developed under low nitrogen concentration. This resistance observed is correlated with low values in oxidative stress indicators (MDA and H2O2) and high values in total phenols.

Read Full Article HTML DOI: 10.29328/journal.jpsp.1001116 Cite this Article Read Full Article PDF

Keywords:

Botrytis cinerea; Sclerotinia minor; Lactuca sativa L; Nitrogen

References

  1. Mengel K, Kirkby EA, Kosegarten H, Appel T. Principles of plant Nutrition. Dordrecht: Kluwer Academic. 2001.
  2. Santamaria P. Nitrate in vegetables: toxicity, content, intake and EC Regulation. J Sci Food Agr. 2006; 86: 10-17.
  3. Addiscott T. Is it nitrate that threatens. NN Cometti, et al.553 Hortic. bras. v.29, n. 4, out. dez. 2011 life or the scare about nitrate? J Sci Food Agr. 2006; 86: 2005-200.
  4. Cometti N, Furlani P. Hydroponics and food security. In: Ferreira A, Lima ABP, Matta FP, Amaral JAT, Lopes JC, Pezzopane JEM, Ferreira MFS, Polanczyk RA, Soares TCB. Special Topics in Plant Production I.1 ed. Alegre ES Center for Agricultural Sciences. 2009; 517-528.
  5. Powlson DS, Addiscott TM, Benjamin N, Cassman KG, de Kok TM, van Grinsven H, L'Hirondel JL, Avery AA, van Kessel C. When does nitrate become a risk for humans? J Environ Qual. 2008 Feb 11;37(2):291-5. doi: 10.2134/jeq2007.0177. PMID: 18268290.
  6. Beninni E, Takahashi H, Neves C, Fonseca I. Nitrate content in lettuce grown in conventional hydroponic systems. Hort Brasileira. 2002; 20: 183-186.
  7. Datnoff LE, Elmer WH, Huber DM. Mineral nutrition and plant disease. American Phytopathological Society (APS Press). St. Paul. 2007.
  8. Huber DM, Thompson IA. Nitrogen and plant disease. In: LE. Datnoff, WH. Elmer DM. Huber (Eds.). Mineral nutrition and plant disease. APS Press, Saint Paul, MN, USA. 2007; 31-44.
  9. Navarrette M, Lecompte F, Collange B, Tchamitchian M. Cultivation systems and quality of lettuce. How to rethink cultivation systems to reduce the use of pesticides and the risks of residues? Innovations Agronomiques. 2010; 9: 67-84.
  10. Lecompte F, Abro MA, Nicot PC. Contrasted responses of Botrytis cinerea islotaes developping on tomato plants grown under different nitrogen nutrition regimes. Plant Pathol. 2010; 59: 891-899.
  11. Verhoeff K. Studies of Botrytis cinerea in tomatoes. Mycelial development in plants growing in soil with various nutrient levels, as well as in internodes of different age. Neth J Plant Pathol. 1965; 71: 167-75.
  12. Hoffland E, Jeger M, Van Beusichem ML. Effect of nitrogen supply rate on disesase resistance in tomato depends on the pathogen. Plant soil. 2000; 218: 239-247.
  13. Hobbs EL, Waters WE. Influence of nitrogen and potassium on susceptibility of Chrysanthemum morifolicum to Botrytis cinerea. Phytopathology. 1964; 54: 674-684.
  14. Sol HH. The effect of different nitrogen sources on (1) the sugars and amino acids leached from leaves and (2) the susceptibility of Vicia faba to attack by Botrytis fabae Meded Fac Landbouwwet Rijksuniv Gent. 1967; 32: 768-775.
  15. Elad Y, Yunis H, Volpin H. Effect of nutrition on susceptibility of cucumber, eggplant and pepper crops to Botrytis cinerea. Can J Bot. 1993; 71: 602-608.
  16. Lecompte F, Abro MA, Nicot PC. Can plant sugars mediate the effect of nitrogen fertilization on lettuce susceptibility to two necrotrophic pathogens: Botrytis cinerea and Sclerotinia sclerotiorum. Plant and Soil. 2013; 369(1-2): 387-401.
  17. Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med. 1976 Apr;15(2):212-6. doi: 10.1016/0006-2944(76)90049-1. PMID: 962904.
  18. Velikova V, Yordancv I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci. 2000; 151: 59-66.
  19. Cicco N, Lanorte M, Paragio M, Viggiano M. A reproductible rapid and inexpensive Folin-Ciocalteu micro- method in determining phenolics of plant methanol extracts. Microchem J. 2009; 91: 107-110.
  20. Duncan DB. Multiple range and multiple F tests. Biometrics. 1955; 11: 1-42.
  21. Hossain MM, Sultana F, Li W, Tran LP, Mostofa MG. Sclerotinia sclerotiorum (Lib.) de Bary: Insights into the Pathogenomic Features of a Global Pathogen. Cells. 2023 Mar 31;12(7):1063. doi: 10.3390/cells12071063. PMID: 37048136; PMCID: PMC10093061.
  22. Ouhibi C, Attia H, Rebah F, Msilini N, Chebbi M, Aarrouf J, Urban L, Lachaal M. Salt stress mitigation by seed priming with UV-C in lettuce plants: growth, antioxidant activity and phenolic compounds. Plant Physiol Biochem. 2014 Oct;83:126-33. doi: 10.1016/j.plaphy.2014.07.019. Epub 2014 Aug 1. PMID: 25133899.
  23. Coria-Cayupán YS, Sánchez de Pinto MI, Nazareno MA. Variations in bioactive substance contents and crop yields of lettuce (Lactuca sativa L.) cultivated in soils with different fertilization treatments. J Agric Food Chem. 2009 Nov 11;57(21):10122-9. doi: 10.1021/jf903019d. PMID: 19821565.
  24. Vries FT, Bloem J, Eekeren NV, Brusaard L. Fungal biomass in pastures increases with age and reduced N input. Soil Biol Biochem. 2007; 39(7):1620–1630.
  25. Vries F, Groenigen JW, Hoffland E, Bloem J. Nitrogen losses from two grassland soils with different fungal biomass. Soil Biol Biochem. 2011; 43(5):997–1005.
  26. Traka S. Contribution to the study of the effect of nitrogen fertilization on the sensitivity of tomatoes to Botrytis cinerea 2019. http://archives.univ-biskra.dz/handle/123456789/13379

Figures:

Similar Articles

Recently Viewed

Read More

Most Viewed

Read More

Help ?