MALDI-MSI method for the detection of large biomolecules in plant leaf tissue
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Abstract
In this study we describe a method for the detection of biomolecules (in the polypeptide m/z range) directly from the surface of plant leaves by using Mass Spectrometry Imaging. The plant-pathogen interaction between Arabidopsis thaliana and the bacterium Xanthomonas campestris pv. campestris was analyzed by comparing infected and non-infected leaf discs submitted to mass spectrometry. The total surface area of ion distribution was calculated for both samples, revealing 23 ions, out of which 3 showed statistical significance. Although these ions were not identified, the results showed that this approach can be successfully applied for the detection of potential polypeptide biomarkers directly on leaf tissue, which is a major challenge in MALDI-Imaging studies.
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Dueñas ME, Feenstra AD, Korte AR, Hinners P, Lee YJ. Cellular and Subcellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution Maldi Mass Spectrometry Imaging. Methods Mol Biol. 2018; 1676: 217-231. PubMed: https://pubmed.ncbi.nlm.nih.gov/28986913/
Solon EG, Schweitzer A, Stoeckli M, Prideaux B. Autoradiography, Maldi-Ms, and Sims-Ms Imaging in Pharmaceutical Discovery and Development. AAPS J. 2010; 12: 11-26. PubMed: https://pubmed.ncbi.nlm.nih.gov/19921438/
Groseclose MR, Andersson M, Hardesty WM, Caprioli RM. Identification of Proteins Directly from Tissue: In Situ Tryptic Digestions Coupled with Imaging Mass Spectrometry. J Mass Spectrometry. 2007; 42: 254-262. PubMed: https://pubmed.ncbi.nlm.nih.gov/17230433/
Michal A, Sabo J, Longuespée R. Microproteomic Sample Preparation. Proteomics. 2021: 2000318. PubMed: https://pubmed.ncbi.nlm.nih.gov/33547857/
Balluff B, Elsner M, Kowarsch A, Rauser S, Meding S, et al. Classification of Her2/Neu Status in Gastric Cancer Using a Breast-Cancer Derived Proteome Classifier. J Prote Res. 2010; 9: 6317-6322. PubMed: https://pubmed.ncbi.nlm.nih.gov/21058730/
Ermini L, Morganti E, Post A, Yeganeh B, Caniggia I, et al. Imaging Mass Spectrometry Identifies Prognostic Ganglioside Species in Rodent Intracranial Transplants of Glioma and Medulloblastoma. PLoS One. 2017; 12: e0176254. PubMed: https://pubmed.ncbi.nlm.nih.gov/28463983/
Ucal Y, Durer ZA, Atak H, Kadioglu E, Sahin B, et al. Ozpinar. Clinical Applications of Maldi Imaging Technologies in Cancer and Neurodegenerative Diseases. Biochimica et Biophysica Acta. 2017; 1865: 795-816. PubMed: https://pubmed.ncbi.nlm.nih.gov/28087424/
Jaegger CF, Negrão F, Assis DM, Belaz KRA, Angolini CFF, et al. Maldi Ms Imaging Investigation of the Host Response to Visceral Leishmaniasis. Mol Biosyst. 2017; 13: 1946-1953. PubMed: https://pubmed.ncbi.nlm.nih.gov/28758666/
Kaspar S, Peukert M, Svatos A, Matros A, Mock HP. Maldi-Imaging Mass Spectrometry - an Emerging Technique in Plant Biology. Proteomics. 2011; 11: 1840-1850. PubMed: https://pubmed.ncbi.nlm.nih.gov/21462348/
Sturtevant D, Lee YJ, Chapman KD. Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (Maldi-Msi) for Direct Visualization of Plant Metabolites in Situ. Curr Opin Biotechnol. 2016; 37: 53-60. PubMed: https://pubmed.ncbi.nlm.nih.gov/26613199/
Araújo FD, Araújo WL, Eberlin MN. Potential of Burkholderia Seminalis Tc3.4.2r3 as Biocontrol Agent against Fusarium Oxysporum Evaluated by Mass Spectrometry Imaging. J Am Society Mass Spectrometry. 2017; 28: 901-907. PubMed: https://pubmed.ncbi.nlm.nih.gov/28194740/
Kim W, Park JJ, Dugan FM, Peever TL, Gang DR, et al. Production of the Antibiotic Secondary Metabolite Solanapyrone a by the Fungal Plant Pathogen Ascochyta Rabiei During Fruiting Body Formation in Saprobic Growth. Environ Microbiol. 2017; 19: 1822-1835. PubMed: https://pubmed.ncbi.nlm.nih.gov/28109049/
Slazak B, Kapusta M, Malik S, Bohdanowicz J, Kuta E, et al. Immunolocalization of Cyclotides in Plant Cells, Tissues and Organ Supports Their Role in Host Defense. Planta. 2016; 244: 1029-1040. PubMed: https://pubmed.ncbi.nlm.nih.gov/27394154/
Klein AT, Yagnik GB, Hohenstein JD, Ji Z, Zi J, et al. Investigation of the Chemical Interface in the Soybean-Aphid and Rice-Bacteria Interactions Using Maldi-Mass Spectrometry Imaging. Anal Chem. 2015; 87: 5294-5301. PubMed: https://pubmed.ncbi.nlm.nih.gov/25914940/
Soares MS, da Silva DS, Forim MR, da Silva MF, Fernandes JB, et al. Machado. Quantification and Localization of Hesperidin and Rutin in Citrus Sinensis Grafted on C. Limonia after Xylella Fastidiosa Infection by Hplc-Uv and Maldi Imaging Mass Spectrometry. Phytochemistry. 2015; 115: 161-170. PubMed: https://pubmed.ncbi.nlm.nih.gov/25749617/
Julia G, Taylor NL, Millar AA. Matrix-Assisted Laser Desorption/Ionisation Mass Spectrometry Imaging and Its Development for Plant Protein Imaging. Plant Methods. 2011; 7: 21. PubMed: https://pubmed.ncbi.nlm.nih.gov/21726462/
Poth AG, Mylne JS, Grassl J, Lyons RE, Millar A, et al. Cyclotides Associate with Leaf Vasculature and Are the Products of a Novel Precursor in Petunia (Solanaceae). J Biol Chem. 2012; 287: 27033-27046. PubMed: https://pubmed.ncbi.nlm.nih.gov/22700981/
Erin G, Keller C, Jayaraman D, Maeda J, Sussman MR, et al. Examination of Endogenous Peptides in Medicago Truncatula Using Mass Spectrometry Imaging. J Prote Res. 2016; 15: 4403-4411. PubMed: https://pubmed.ncbi.nlm.nih.gov/27726374/
Eriksson C, Masaki N, Yao I, Hayasaka T, Setou M. Maldi Imaging Mass Spectrometry-a Mini Review of Methods and Recent Developments. Mass Spectrometry (Tokyo). 2013; 2: S0022. PubMed: https://pubmed.ncbi.nlm.nih.gov/24349941/
Longuespée R, Casadonte R, Kriegsmann M, Pottier C, Picard de Muller G, et al. Maldi Mass Spectrometry Imaging: A Cutting-Edge Tool for Fundamental and Clinical Histopathology. Proteomics Clin Appl. 2016; 10: 701-719. PubMed: https://pubmed.ncbi.nlm.nih.gov/27188927/
Attia Ahmed S, Schroeder KA, Seeley EH, Wilson KJ, Hammer ND, et al. Monitoring the Inflammatory Response to Infection through the Integration of Maldi Ims and Mri. Cell Host & Microbe. 2010; 11: 664-673. PubMed: https://pubmed.ncbi.nlm.nih.gov/22704626/
Alves BE, Bonfim MF, Bloch C, Engler G, Rocha T, et al. Imaging Mass Spectrometry of Endogenous Polypeptides and Secondary Metabolites from Galls Induced by Root-Knot Nematodes in Tomato Roots. Molecular Plant-Microbe Interactions. 2018; 31: 1048-1059. PubMed: https://pubmed.ncbi.nlm.nih.gov/29663868/
Boughton Berin A, Thinagaran D, Sarabia D, Bacic A, Roessner U. Mass Spectrometry Imaging for Plant Biology: A Review. Phytochem Rev. 2016; 15: 445-488. PubMed: https://pubmed.ncbi.nlm.nih.gov/27340381/
Yonghui D, Li B, Malitsky S, Rogachev I, Aharoni A, et al. Sample Preparation for Mass Spectrometry Imaging of Plant Tissues: A Review. Front Plant Sci. 2016; 7: 60. PubMed: https://pubmed.ncbi.nlm.nih.gov/26904042/
Nanna B, Li B, D'Alvise J, Janfelt C. Mass Spectrometry Imaging of Plant Metabolites – Principles and Possibilities. Nat Prod Rep. 2014; 31: 818-837. PubMed: https://pubmed.ncbi.nlm.nih.gov/24452137/
Debois D, Jourdan E, Smargiasso N, Thonart P, De Pauw E, et al. Spatiotemporal Monitoring of the Antibiome Secreted by Bacillus Biofilms on Plant Roots Using Maldi Mass Spectrometry Imaging. Anal Chem. 2014; 86: 4431-4438. PubMed: https://pubmed.ncbi.nlm.nih.gov/24712753/